Resins for Plasma Protein Purification

Heparin Resins
Heparin affinity chromatography is used in the manufacture of several plasma factors, such as Factor VIII, IX, vWF and ATIII.  Heparin Actigel has been used in the fractionation of plasma products for the manufacture of licensed therapeutic proteins for many years.  The resins are stable in 0.5M NaOH, 6M guanidine HCl, 8M urea, and some organic solvents.  Drug Master Files have been filed with the FDA.

Heparin Actigel
Heparin Actigel utilizes a USP grade porcine heparin ligand for plasma fractionation at low pressures and flow rates. The resin has negligible ligand leaching, and is readily sanitized with sodium hydroxide.
Product Code: 37304    Download:      >>Specification Sheet     >>Instructions for Use      >>Safety Data Sheet

Heparin Superflow
Heparin Superflow is similar to Heparin Actigel, but uses a hardened agarose base that allows for use under higher pressures and flow rates.
Product Code: 37304S  Download:      >>Specification Sheet     >>Instructions for Use      >>Safety Data Sheet
 

Dextran Sulfate Resins
Sterogene manufactures resins with a Dextran Sulfate ligand for the isolation of Factor IX from plasma. These resins can also be used in the purification of recombinant plasma protein.
DS- Superflow, DS-Cellthru  >>Inquire
 

Chelating Resins
Sterogene Bioseparations is a leading manufacturer of resins featuring ligands with chelating moieties. Immobilized Metal Affinity Chromatography (IMAC) is used in the production of several plasma proteins, such as Factors IX and X, alpha1-proteinase inhibitor as well as C1 Inhibitor. The resins are stable in 0.5M NaOH, chaotropes like 6M guanidine HCl or 8M urea, and some organic solvents. 
The resins can be charged with any metal.

IDA Chelating CellthruTM BigBead
IDA Chelating CellthruTM BigBead is an uncharged IMAC resin, which allows the customer to select a metal ion for optimizing their separation. This also features Sterogene’s patented Direct Capture™ technology to capture target molecules from a feed stream containing particles without having to filter or centrifuge beforehand.
Product Code: 37311     Download:    >>Specification Sheet    >>Instructions for Use    >>Safety Data Sheet

IDA Chelating Superflow
IDA Chelating  Superflow is a highly crosslinked IMAC resin that does not compress at flow rates used for medium- to large-scale purifications (linear flow rates up to 1200cm/hour). The resin is compatible with a variety of chemicals and pH values and with common clean-in-place procedures. The resin is stable through multiple cycles of cleaning and reuse. Recommended for purification of polyhistidine tagged proteins.
Product Code: 37410      Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet
 

Benzamidine Resin
Benzamidine Superflow features a p-aminobenzamidine ligand, an  inhibitor of trypsin-like serine proteases, covalently attached to a 6% agarose support resin. Trypsin and trypsin-like serine proteases bind to Benzamidine  allowing for purification and/ or removal of these proteins. Benzamidine affinity resin is used in the purification of native plasminogen and plasmin.   >>Inquire
 

Lysine Resin
Lysine Superflow features L-lysine immobilized to 4% highly crosslinked agarose. Lysine resin is an affinity adsorbent and can be used for isolation of plasminogen and the plasminogen activator, alpha1-proteinase inhibitor.   >>Inquire
 

Lectin Affinity Resins
Lectins are proteins that interact specifically and reversibly with certain sugar residues on proteins. This specificity enables binding to polysaccharides and glycoproteins. Immobilized lectins, such as ConA Superflow and Jacalin Superflow can be used for the purification of several plasma proteins, such as alpha1-proteinase inhibitor and C1 Inhibitor.  >>Inquire
 

IonClear BigBeadTM
IonClear BigBeadsTM are 500 – 1,100 micron diameter beads with both cationic and anionic chains covalently linked to a polystyrene sphere. This resin rapidly removes both anions and cations from solutions with minimal binding of proteins.
Product Code: 30041       Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet
 

Immunoaffinity Purification of plasma proteins
Actigel ALD  resins are available with an  included coupling solution, for the immobilization of proteins, peptides, or other ligands containing primary amines.  The coupling is gentle on the ligand, extremely efficient, and scales up reproducibly.  The resulting secondary amine is stable, even in 0.15 M NaOH, providing the ligand can withstand such harsh treatment. Leaching from the resin is below the limit of detection. Actigel ALD is used in the production of licensed biopharmaceuticals, including plasma proteins, and Sterogene has a Drug Master File with the FDA.

Actigel ALD
Actigel ALD is a cross-linked agarose bead with aldehyde functional groups covalently bound to the matrix.  The carbonyl moiety is useful for stable linkage of peptides or proteins. Actigel ALD is an excellent all-purpose resin due to its stability and resistance to alkali.
Product Code: 2701       Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet

Actigel ALD Superflow
Actigel ALD Superflow is a cross-linked agarose  matrix with aldehyde functional groups. The carbonyl moeity is useful for stable linkage of peptides or proteins.  Actigel ALD Superflow is designed for high flow rates and is an excellent all-purpose resin due to its stability and resistance to alkali.
Product Code: 2701S      Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet
 

Endotoxin Removal Resin

ActiClean Etox
ActiClean Etox efficiently removes endotoxins from plasma proteins, including albumin, factor VIII, factor IX and IVIG solutions with minimal non-specific binding. The immobilized ligand is non-toxic and leaching is negligible. ActiClean Etox is easily regenerated for re-use. There is a Drug Master File with the FDA. This product has been used in the manufacture of licensed therapeutics.
Product Code: 2705       Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet

ReductEtox
ReductEtox is designed to easily and rapidly remove endotoxins from proteins and plasmid DNA, and is easily sanitized and regenerated.
Product Code: 2709      Download:      >>Specification Sheet     >>Instructions for Use     >>Safety Data Sheet